That's my opinion, but would like confirmation. Do these have layers?

Hi Rosanna, no there are no layers. Why do you think they are aspeo? Judy

My understanding is that the gently rounded ends and slumped shapes are a result of re-heating the bead on a spit (speo). Maybe this can be done without the spit, not sure. How big are the holes? are the holes collapsed and irregular or round and regular?

Origin from the Netherlands (Amsterdam, Middelburg) is possible too
And maybe not a Speo, but pinched (the hot glass tube cut with a scissor, rotating)
Like to see pictures of the hole, is there by some a kind of rim around it?
hans

Hi Judy,
sorry I didn't see your response earlier. I can't tell by your last photograph if the beads are pinched. But, if they had a "a speo" treatment the surface would be shinier, more dense and globular. I think...

Hans

Jamey Allen's opinion regarding these beads is as follows:-
Rosanna's reply to your question is correct. These are not hot-pinched beads. However, it's not impossible they were made in Holland or England, at the same time such beads were made at Venice. From the 17th C.

It is also true that, when new, a-speo beads would have been shiny (unless treated to not be shiny). But, MANY beads from an African context have lost their shine, a long time ago. Africa, as an environment, is unkind to glass.